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The main elements of the HPLC are revealed in Figure (PageIndex three ). The position of the pump will be to power a liquid (cell section) through at a particular circulation level (milliliters per minute). The injector serves to introduce the liquid sample in the move stream with the mobile stage. Column is the most central and essential part of H
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Add a known amount of the antidepressant protriptyline, which serves as an inside conventional, to each serum sample and to every exterior conventional. To eliminate matrix interferents, pass a 0.five-mL aliquot of every serum sample or common via a C18 reliable-phase extraction cartridge. Just after washing the cartridge to get rid of the interfer